Virological assessment of fetuses and neonates

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West of Scotland  SPECIALIST VIROLOGY CENTRE
Level 5 New Lister Building, Glasgow Royal Infirmary, 10-16 Alexandra Parade, Glasgow, G31 2ER

General

Contacting Laboratory

  • Weekdays 9am-5pm

    0141 201 8722 (enquiries/results)

    Weekends and out of hours (24 hrs) contact on call medical virologist via GRI switchboard ( 0141 211 4000  )
  • There are only a limited number of tests available out of hours.
  • Routine samples are delivered to the laboratory by porter/ pod system/ via other labs.
  • For urgent samples during working hours phone the lab and arrange a taxi. Check when the results are likely to be available and provide us with contact details.
  • All authorised results are available via SCI store or clinical portal providing the CHI number is included on the request form.
  • Swabs should be placed in VPSS or VTM. VPSS has a long shelf life at room temperature and is suitable for all types of swab including eye swabs (chlamydia).  Use a dry swab, squeeze the swabbed material into VPSS or VTM by pressing against the side of the vial and discard the swab.  Do not leave the swab in the medium.  VPSS is an irritant and should not come into contact with the baby
  • VPSS can be obtained directly from us or via local microbiology service.
Samples
  1. Always provide clinical details including age, gestation, date of onset of symptoms and any fetal abnormalities (if relevant). This helps with test selection.
  2. We no longer perform TORCH screens or test babies with a diagnosis of conjugated hyperbilirubinaemia. Phone the lab to discuss.
  3. It is helpful to provide the mother’s name and date of birth. Testing of antenatal samples may help confirm or rule out a specific infection.
  4. Routine testing of maternal or fetal samples from IUD cases is rarely helpful. If a specific pathogen is suspected please write this on the form and send with a small piece of placental tissue (not swab).
  5. Amniotic fluid / fetal blood samples can be tested but not until at least 6 weeks post acute illness, and not before 18-20 weeks gestation.
  6. As a general principle always take a sample from the affected system.
Prenatal/Postnatal Infections
  • Classical features of in utero infections include the following:
    CMV Hepatosplenomegaly, microcephaly, chorioretinitis, cerebral  calcification,   thrombocytopaenia. (see WoS guideline for management)    
    Rubella Cardiac, ocular and neurological defects, hepatosplenomegaly, purpura. This is extremely rare.
    Toxoplasma Hydrocephalus, retinochoroiditis, hepatitis, pneumonia, myocarditis
    Erythrovirus B19

    Fetal anaemia / hydrops 

    (B19 is not associated with congenital malformation)

  • Congenital CMV is diagnosed by sending a urine +/- saliva for CMV PCR testing.    This must be done within 21 days of life
  • To diagnose HIV infection, see WoS policy or discuss with virology.
  • Testing for HBV or HCV in the under 1’s is not recommended as maternal antibodies may still be present. Please discuss with virologist if testing is required.
  • If HSV disease is suspected send swabs, urine and blood +/- CSF. Surface swabs and a blood are required if the baby has been exposed but is not showing signs of infection. (see WoS guideline)
Recent Infection (Postnatal)
  1. Diagnosis relies on samples taken from the system(s) involved (see table).
  2. Most tests use PCR technology, these take an average 3-5 hours to complete from arrival in the lab. Most of the tests are multiplexed.  This means that a sample may be tested for more viruses than you requested

Respiratory Screen by PCR  (2 parts)
RS1 – influenza A/B, RSV, adenovirus, mycoplasma
RS2 - parainfluenza 1-4, coronaviruses, rhinovirus, human metapneumovirus. 

In addition, CMV is tested on the respiratory secretions of all babies <21 days of age & Chlamydia is tested on all negative samples in babies (<2month age).
(NB- if Pertussis PCR testing is required this must be requested separately)

Blood – CMV, EBV, adenovirus, HSV
CSF – HSV1/2, VZV, enterovirus.
Skin swab – HSV/VZV.
Stool samples – norovirus, rotavirus, sapovirus, astrovirus, adenovirus
Eyes – HSV, VZV, Adenovirus, Chlamydia trachomatis
  1. In situations which have infection control implications (e.g. outbreak or VZ contact), please contact the lab and infection control to coordinate testing.
Neonatal guide to virology tests (Feb 2014)

** Samples are from the neonate unless otherwise specified

Editorial Information

Last reviewed: 18 June 2018

Next review: 01 July 2021

Author(s): Dr C Aiken, Consultant Virologist

Approved By: MCN for Neonatology, West of Scotland

Reviewer Name(s): Dr Celia Jackson, Consultant Virologist